alpha Defensin 1 (DEFA1) (NM_004084) Human Mass Spec Standard

CAT#: PH318421

DEFA1 MS Standard C13 and N15-labeled recombinant protein (NP_004075)

Product Images

Coomassie blue staining of purified DEFA1 protein (Cat# TP318421). The protein was produced from HEK293T cells transfected with DEFA1 cDNA clone (Cat# RC218421) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC218421
• Predicted MW: 10 kDa
• Protein Sequence:
  Sequence Link

  >RC218421 representing NM_004084
  Red=Cloning site Green=Tags(s)
  
  MRTLAILAAILLVALQAQAEPLQARADEVAAAPEQIAADIPEVVVSLAWDESLAPKHPGSRKNMDCYCRI
  PACIAGERRYGTCIYQGRLWAFCC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_004075
• RefSeq Size: 490
• RefSeq ORF: 282
• Synonyms: DEF1; DEFA2; HNP-1; HP-1; HP1; MRS
• Locus ID: 1667
• UniProt ID: P59665
• Cytogenetics: 8p23.1
• Summary: 'Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. [provided by RefSeq, Oct 2014]'
• Protein Families: Druggable Genome, Secreted Protein