ISM1 (NM_080826) Human Mass Spec Standard

CAT#: PH322172

ISM1 MS Standard C13 and N15-labeled recombinant protein (NP_543016)

Product Images

Coomassie blue staining of purified ISM1 protein (Cat# TP322172). The protein was produced from HEK293T cells transfected with ISM1 cDNA clone (Cat# RC222172) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC222172
• Predicted MW: 51.9 kDa
• Protein Sequence:
  Sequence Link

  >RC222172 representing NM_080826
  Red=Cloning site Green=Tags(s)
  
  MVRLAAELLLLLGLLLLTLHITVLRGSGAADGPDAAAGNASQAQLQNNLNVGSDTTSETSFSLSKEAPRE
  HLDHQAAHQPFPRPRFRQETGHPSLQRDFPRSFLLDLPNFPDLSKADINGQNPNIQVTIEVVDGPDSEAD
  KDQHPENKPSWSVPSPDWRAWWQRSLSLARANSGDQDYKYDSTSDDSNFLNPPRGWDHTAPGHRTFETKD
  QPEYDSTDGEGDWSLWSVCSVTCGNGNQKRTRSCGYACTATESRTCDRPNCPGIEDTFRTAATEVSLLAG
  SEEFNATKLFEVDTDSCERWMSCKSEFLKKYMHKVMNDLPSCPCSYPTEVAYSTADIFDRIKRKDFRWKD
  ASGPKEKLEIYKPTARYCIRSMLSLESTTLAAQHCCYGDNMQLITRGKGAGTPNLISTEFSAELHYKVDV
  LPWIICKGDWSRYNEARPPNNGQKCTESPSDEDYIKQFQEAREY
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_543016
• RefSeq Size: 2610
• RefSeq ORF: 1392
• Synonyms: bA149I18.1; C20orf82; dJ1077I2.1; ISM; Isthmin
• Locus ID: 140862
• UniProt ID: B1AKI9
• Cytogenetics: 20p12.1