UBE2I (NM_194260) Human Mass Spec Standard

CAT#: PH324182

UBE2I MS Standard C13 and N15-labeled recombinant protein (NP_919236)

Product Images

Coomassie blue staining of purified UBE2I protein (Cat# TP324182). The protein was produced from HEK293T cells transfected with UBE2I cDNA clone (Cat# RC224182) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC224182
• Predicted MW: 17.8 kDa
• Protein Sequence:
  Sequence Link

  >RC224182 representing NM_194260
  Red=Cloning site Green=Tags(s)
  
  MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPS
  SPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQN
  RVEYEKRVRAQAKKFAPS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_919236
• RefSeq Size: 1144
• RefSeq ORF: 474
• Synonyms: C358B7.1; P18; UBC9
• Locus ID: 7329
• UniProt ID: P63279
• Cytogenetics: 16p13.3
• Summary: 'The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]'
• Protein Pathways: Ubiquitin mediated proteolysis