NT5C1A (NM_032526) Human Mass Spec Standard

CAT#: PH324617

NT5C1A MS Standard C13 and N15-labeled recombinant protein (NP_115915)

Product Images

Coomassie blue staining of purified NT5C1A protein (Cat# TP324617). The protein was produced from HEK293T cells transfected with NT5C1A cDNA clone (Cat# RC224617) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC224617
• Predicted MW: 41.5 kDa
• Protein Sequence:
  Sequence Link

  >RC224617 representing NM_032526
  Red=Cloning site Green=Tags(s)
  
  MEPGQPREPQEPREPGPGAETAAAPVWEEAKIFYDNLAPKKKPKSPKPQNAVTIAVSSRALFRMDEEQQI
  YTEQGVEEYVRYQLEHENEPFSPGPAFPFVKALEAVNRRLRELYPDSEDVFDIVLMTNNHAQVGVRLINS
  INHYDLFIERFCMTGGNSPICYLKAYHTNLYLSADAEKVREAIDEGIAAATIFSPSRDVVVSQSQLRVAF
  DGDAVLFSDESERIVKAHGLDRFFEHEKAHENKPLAQGPLKGFLEALGRLQKKFYSKGLRLECPIRTYLV
  TARSAASSGARALKTLRSWGLETDEALFLAGAPKGPLLEKIRPHIFFDDQMFHVAGAQEMGTVAAHVPYG
  VAQTPRRTAPAKQAPSAQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_115915
• RefSeq Size: 1107
• RefSeq ORF: 1104
• Synonyms: CN-I; CN-IA; CN1; CN1A; CNI
• Locus ID: 84618
• UniProt ID: Q9BXI3
• Cytogenetics: 1p34.2
• Summary: Cytosolic nucleotidases, such as NT5C1A, dephosphorylate nucleoside monophosphates (Hunsucker et al., 2001 [PubMed 11133996]). [supplied by OMIM, Mar 2008]
• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism, Purine metabolism, Pyrimidine metabolism