FLIP (CFLAR) (NM_001127184) Human Mass Spec Standard

CAT#: PH325270

CFLAR MS Standard C13 and N15-labeled recombinant protein (NP_001120656)

Product Images

Coomassie blue staining of purified CFLAR protein (Cat# TP325270). The protein was produced from HEK293T cells transfected with CFLAR cDNA clone (Cat# RC225270) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC225270
• Predicted MW: 25.2 kDa
• Protein Sequence:
  Sequence Link

  >RC225270 representing NM_001127184
  Red=Cloning site Green=Tags(s)
  
  MSAEVIHQVEEALDTDEKEMLLFLCRDVAIDVVPPNVRDLLDILRERGKLSVGDLAELLYRVRRFDLLKR
  ILKMDRKAVETHLLRNPHLVSDYRVLMAEIGEDLDKSDVSSLIFLMKDYMGRGKISKEKSFLDLVVELEK
  LNLVAPDQLDLLEKCLKNIHRIDLKTKIQKYKQSVQGAGTSYRNVLQAAIQKSLKDPSNNFRMITPYAHC
  PDLKILGNCSM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001120656
• RefSeq ORF: 663
• Synonyms: c-FLIP; c-FLIPL; c-FLIPR; c-FLIPS; CASH; CASP8AP1; Casper; cFLIP; CLARP; FLAME; FLAME-1; FLAME1; FLIP; I-FLICE; MRIT
• Locus ID: 8837
• UniProt ID: O15519
• Cytogenetics: 2q33.1
• Summary: The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011]
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Apoptosis