Bcl2 Binding component 3 (BBC3) (NM_001127240) Human Mass Spec Standard

CAT#: PH325345

BBC3 MS Standard C13 and N15-labeled recombinant protein (NP_001120712)

Product Images

Coomassie blue staining of purified BBC3 protein (Cat# TP325345). The protein was produced from HEK293T cells transfected with BBC3 cDNA clone (Cat# RC225345) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC225345
• Predicted MW: 26.3 kDa
• Protein Sequence:
  Sequence Link

  >RC225345 representing NM_001127240
  Red=Cloning site Green=Tags(s)
  
  MKFGMGSAQACPCQVPRAASTTWVPCQICGPRERHGPRTPGGQLPGARRGPGPRRPAPLPARPPGALGSV
  LRPLRARPGCRPRRPHPAARCLPLRPHRPTRRHRRPGGFPLAWGSPQPAPRPAPGRSSALALAGGAAPGV
  ARAQRPGGSGGRSHPGGPGSPRGGGTVGPGDRGPAAADGGRPQRTVRAAETRGAAAAPPLTLEGPVQSHH
  GTPALTQGPQSPRDGAQLGACTRPVDVRDSGGRPLPPPDTLASAGDFLCTM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001120712
• RefSeq ORF: 783
• Synonyms: JFY-1; JFY1; PUMA
• Locus ID: 27113
• UniProt ID: Q9BXH1, Q96PG8
• Cytogenetics: 19q13.32
• Summary: This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]
• Protein Families: Druggable Genome
• Protein Pathways: Huntington's disease, p53 signaling pathway