JMJD5 (KDM8) (NM_001145348) Human Mass Spec Standard

CAT#: PH327709

JMJD5 MS Standard C13 and N15-labeled recombinant protein (NP_001138820)

Product Images

Coomassie blue staining of purified KDM8 protein (Cat# TP327709). The protein was produced from HEK293T cells transfected with KDM8 cDNA clone (Cat# RC227709) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC227709
• Predicted MW: 50.7 kDa
• Protein Sequence:
  Sequence Link

  >RC227709 representing NM_001145348
  Red=Cloning site Green=Tags(s)
  
  MSREKCSPGEGAEEGRGSEASGLKASAGHGTEPAGGGPMAGDTHCPAEPLAREGTLWEALRALLPHSKED
  LKLDLGEKVERSVVTLLQRATELFYEGRRDECLQSSEVILDYSWEKLNTGTWQDVDKDWRRVYAIGCLLK
  ALCLCQAPEDANTVAAALRVCDMGLLMGAAILGDILLKVAAILQTHLPGKRPARGSLPEQPCTKKARADH
  GLIPDVKLEKTVPRLHRPSLQHFREQFLVPGRPVILKGVADHWPCMQKWSLEYIQEIAGCRTVPVEVGSR
  YTDEEWSQTLMTVNEFISKYIVNEPRDVGYLAQHQLFDQIPELKQDISIPDYCSLGDGEEEEITINAWFG
  PQGTISPLHQDPQQNFLVQVMGRKYIRLYSPQESGALYPHDTHLLHNTSQVDVENPDLEKFPKFAKAPFL
  SCILSPGEILFIPVKYWHYVRALDLSFSVSFWWS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001138820
• RefSeq ORF: 1362
• Synonyms: JMJD5
• Locus ID: 79831
• UniProt ID: Q8N371
• Cytogenetics: 16p12.1
• Summary: This gene likely encodes a histone lysine demethylase. Studies of a similar protein in mouse indicate a potential role for this protein as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2009]