NDE1 (NM_001143979) Human Mass Spec Standard

CAT#: PH327919

NDE1 MS Standard C13 and N15-labeled recombinant protein (NP_001137451)

Product Images

Coomassie blue staining of purified NDE1 protein (Cat# TP327919). The protein was produced from HEK293T cells transfected with NDE1 cDNA clone (Cat# RC227919) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC227919
• Predicted MW: 37.7 kDa
• Protein Sequence:
  Sequence Link

  >RC227919 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEDSGKTFSSEEEEANYWKDLAMTYKQRAENTQEELREFQEGSREYEAELETQLQQIETRNRDLLSENNR
  LRMELETIKEKFEVQHSEGYRQISALEDDLAQTKAIKDQLQKYIRELEQANDDLERAKRATIMSLEDFEQ
  RLNQAIERNAFLESELDEKENLLESVQRLKDEARDLRQELAVQQKQEKPRIPMPSSVEAERTDTAVQATG
  SVPSTPIAHRGPSSSLNTPGSFRRGLDDSTGGTPLTPAARISALNIVGDLLRKVGALESKLASCRNLVYD
  QSPNRTGGPASGRSSKNRDGGERRPSSTSVPLGDKGLDTSCRWLSKSTTRSSSSC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: 50 ug/ml as determined by BCA
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001137451
• RefSeq Size: 3936
• RefSeq ORF: 1005
• Synonyms: HOM-TES-87; LIS4; MHAC; NDE; NUDE; NUDE1
• Locus ID: 54820
• UniProt ID: Q9NXR1, X5DR54
• Cytogenetics: 16p13.11
• Summary: This gene encodes a member of the nuclear distribution E (NudE) family of proteins. The encoded protein is localized at the centrosome and interacts with other centrosome components as part of a multiprotein complex that regulates dynein function. This protein plays an essential role in microtubule organization, mitosis and neuronal migration. Mutations in this gene cause lissencephaly 4, a disorder characterized by lissencephaly, severe brain atrophy, microcephaly, and severe cognitive disability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2012]