Alkaline phosphatase / PLAP / ALPP Human Protein

CAT#: BA027

Alkaline phosphatase / PLAP / ALPP human protein, 0.1 kU

Specifications

Product Data

• Species: Human
• Protein Source: Placenta
• Purity: 750U/mg 95% protein (by Biuret).
• Buffer: State: Lyophilized
• Bioactivity: Specific: 1125 U/mg protein, 1069 U/mg solid
  One unit liberates one micromole of p-nitrophenol per minute at 37°C, pH 10.15 in Tris buffer.
• Reconstitution: Restore with sterile water or dilution buffer.
• Preparation: Lyophilized
• Applications: ELISA.
• Protein Description: Purified native human Alkaline Phosphatase (AP)
• Note: Caution: Source material supplied to your facility has been tested negative for Hepatitis and HIV. Nevertheless, all products from human sources should be handled as potentially infectious.
• Storage: Prior to and following reconstitution store (undiluted) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_001623
• Locus ID: 250
• Cytogenetics: 2q37.1
• Synonyms: ALP; ALPI; IAP; PALP; PLAP; PLAP-1
• Summary: 'The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. One of the main sources of this enzyme is the liver, and thus, it's one of several indicators of liver injury in different clinical conditions. In pregnant women, this protein is primarily expressed in placental and endometrial tissue, however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. [provided by RefSeq, Aug 2020]'
• Protein Pathways: Folate biosynthesis, Metabolic pathways