MITF (NM_198178) Human Over-expression Lysate
CAT#: LC404984
MITF HEK293T cell transient overexpression lysate (as WB positive control)
Size: 100 ug
Specifications
Product Data | |
Species | Human |
Applications | WB |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence | TrueORF Clone RC222074 |
Tag | C-Myc/DDK |
Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
Accession Number | NM_198178, NP_937821 |
Other Names | bHLHe32; CMM8; COMMAD; MI; WS2; WS2A |
Predicted MW | 55.2 kDa |
Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
Reference Data | |
RefSeq | NP_937821 |
Locus ID | 4286 |
Cytogenetics | 3p13 |
Protein Families | Druggable Genome, Transcription Factors |
Protein Pathways | Melanogenesis, Melanoma, Pathways in cancer |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Recombinant Protein Resources |
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
LC424990 | MITF HEK293T cell transient overexpression lysate (as WB positive control) |
USD 121.00 |
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USD 396.00 |
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TP761396 | Purified recombinant protein of Human microphthalmia-associated transcription factor (MITF), transcript variant 5, full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug |
USD 215.00 |
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TP761494 | Purified recombinant protein of Human microphthalmia-associated transcription factor (MITF), transcript variant 4, full length, with N-terminal HIS tag, expressed in E.coli, 50ug |
USD 215.00 |
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