HES1 (NM_005524) Human Over-expression Lysate

CAT#: LY417251

Transient overexpression lysate of hairy and enhancer of split 1, (Drosophila) (HES1)

Size: 20 ug 100 ug


  View other "HES1" proteins (4)

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USD 396.00

In Stock*

Size
    • 100 ug

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Frequently bought together (3)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
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HES1 mouse monoclonal antibody, clone OTI2D2 (formerly 2D2)
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beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control
    • 30 ul

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Other products for "HES1"

Specifications

Product Data
Species Human
Applications WB
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC211709
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
Accession Number NM_005524, NP_005515
Other Names bHLHb39; HES-1; HHL; HRY
Predicted MW 29.4 kDa
Components 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Storage The lysate is shipped with dry ice. The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_005515
Locus ID 3280
Cytogenetics 3q29
Protein Families Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transcription Factors
Protein Pathways Maturity onset diabetes of the young, Notch signaling pathway

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