Superoxide Dismutase 1 / SOD1 (1-154) Human Protein

CAT#: AR03033PU-L

Superoxide Dismutase 1 / SOD1 (1-154) human recombinant protein, 0.5 mg


USD 845.00

2 Weeks*

Size
    • 500 ug

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Other products for "SOD1"

Specifications

Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
MATKAVCVLK GDGPVQGIIN FEQKESNGPV KVWGSIKGLT EGLHGFHVHE FGDNTAGCTS AGPHFNPLSR KHGGPKDEER HVGDLGNVTA DKDGVADVSI EDSVISLSGD HCIIGRTLVV HEKADDLGKG GNEESTKTGN AGSRLACGVI GIAQ
Predicted MW 15.9 kDa
Concentration lot specific
Purity >95% pure by SDS-PAGE
Buffer Presentation State: Purified
State: Liquid purified protein
Buffer System: 20 mM Tris pH 7.5, 10% Glycerol
Bioactivity Biological: Specific activity is >1,000 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7. 5 at 25°C.
Endotoxin <1.0 EU per 1 microgram of protein (determined by LAL method)
Preparation Liquid purified protein
Applications Protocol: Activity Assay
1. Prepare a 1.5 ml reaction mix into a suitable container and pre-chill on ice before use: The final concentrations are 50 mM potassium phosphate, 0.1 mM ethylendiaminetetraacetic acid, 0.01 mM cytochrom C, 0.05 mM xanthine, 0.005 units xanthine oxidase.
2. Equilibrate to 25°C and monitor at A550nm until the value is constant using a spectrophotometer.
3. Add 50 ul of recombinant SOD protein in various concentrations (0.5ug, 1ug) in assay buffer.
4. Mix by inversion and record the increase at A550nm for 5 minutes.
Protein Description Recombinant SOD1 was expressed in E.coli and purified by conventional chromatography techniques.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Reference Data
RefSeq NP_000445
Locus ID 6647
UniProt ID P00441, V9HWC9
Cytogenetics 21q22.11
Synonyms ALS; ALS1; HEL-S-44; homodimer; hSod1; IPOA; SOD; STAHP
Summary 'The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. In addition, this protein contains an antimicrobial peptide that displays antibacterial, antifungal, and anti-MRSA activity against E. coli, E. faecalis, S. aureus, S. aureus MRSA LPV+, S. agalactiae, and yeast C. krusei. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2020]'
Protein Families Druggable Genome
Protein Pathways Amyotrophic lateral sclerosis (ALS), Huntington's disease, Prion diseases

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.