Superoxide Dismutase 1 / SOD1 (1-154) Human Protein

CAT#: AR03033PU-L

Superoxide Dismutase 1 / SOD1 (1-154) human recombinant protein, 0.5 mg

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MATKAVCVLK GDGPVQGIIN FEQKESNGPV KVWGSIKGLT EGLHGFHVHE FGDNTAGCTS AGPHFNPLSR KHGGPKDEER HVGDLGNVTA DKDGVADVSI EDSVISLSGD HCIIGRTLVV HEKADDLGKG GNEESTKTGN AGSRLACGVI GIAQ
• Predicted MW: 15.9 kDa
• Concentration: lot specific
• Purity: >95% pure by SDS-PAGE
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: 20 mM Tris pH 7.5, 10% Glycerol
• Bioactivity: Biological: Specific activity is >1,000 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7. 5 at 25°C.
• Endotoxin: <1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation: Liquid purified protein
• Applications: Protocol: Activity Assay
  1. Prepare a 1.5 ml reaction mix into a suitable container and pre-chill on ice before use: The final concentrations are 50 mM potassium phosphate, 0.1 mM ethylendiaminetetraacetic acid, 0.01 mM cytochrom C, 0.05 mM xanthine, 0.005 units xanthine oxidase.
  2. Equilibrate to 25°C and monitor at A550nm until the value is constant using a spectrophotometer.
  3. Add 50 ul of recombinant SOD protein in various concentrations (0.5ug, 1ug) in assay buffer.
  4. Mix by inversion and record the increase at A550nm for 5 minutes.
• Protein Description: Recombinant SOD1 was expressed in E.coli and purified by conventional chromatography techniques.
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_000445
• Locus ID: 6647
• UniProt ID: P00441, V9HWC9
• Cytogenetics: 21q22.11
• Synonyms: ALS; ALS1; HEL-S-44; homodimer; hSod1; IPOA; SOD; STAHP
• Summary: 'The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. In addition, this protein contains an antimicrobial peptide that displays antibacterial, antifungal, and anti-MRSA activity against E. coli, E. faecalis, S. aureus, S. aureus MRSA LPV+, S. agalactiae, and yeast C. krusei. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2020]'
• Protein Families: Druggable Genome
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Huntington's disease, Prion diseases