Thioredoxin-2 / TRX2 (60-166) Human Protein

CAT#: AR09090PU-L

Thioredoxin-2 / TRX2 (60-166) human recombinant protein, 0.5 mg

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MTTFNIQDGP DFQDRVVNSE TPVVVDFHAQ WCGPCKILGP RLEKMVAKQH GKVVMAKVDI DDHTDLAIEY EVSAVPTVLA MKNGDVVDKF VGIKDEDQLE AFLKKLIG
• Concentration: lot specific
• Purity: ≥95 by SDS PAGE
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: PBS, pH 7.4
• Bioactivity: Specific: 3-4 A650/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
• Preparation: Liquid purified protein
• Applications: Protocol: Activity Assay
  1. Prepare a 1 ml reaction cocktail into a suitable container : The final concentrations are 5mM Tris, 63mM sodium phosphate, 1mM DTT, 0.1% (w/v) insulin, 2mM EDTA.
  2. Add 4 ug of recombinant thioredoxin2 into reaction cocktail and mix immediately.
  3. Incubate at 25°C for 20 minutes.
  4. Record the increase in A650nm for 15 minutes.
• Protein Description: Recombinant Thioredoxin2 was expressed in E.coli and purified by using conventional chromatography techniques.
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_036605
• Locus ID: 25828
• UniProt ID: Q99757
• Cytogenetics: 22q12.3
• Synonyms: COXPD29; MT-TRX; MTRX; TRX2; TXN
• Summary: This nuclear gene encodes a mitochondrial member of the thioredoxin family, a group of small multifunctional redox-active proteins. The encoded protein may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis. [provided by RefSeq, Jul 2008]
• Protein Families: Druggable Genome