TWIST2 (NM_057179) Human Recombinant Protein

CAT#: TP305006

Recombinant protein of human twist homolog 2 (Drosophila) (TWIST2)

Product Images

Coomassie blue staining of purified TWIST2 protein (Cat# TP305006). The protein was produced from HEK293T cells transfected with TWIST2 cDNA clone (Cat# RC205006) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >RC205006 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEEGSSSPVSPVDSLGTSEEELERQPKRFGRKRRYSKKSSEDGSPTPGKRGKKGSPSAQSFEELQSQRIL
  ANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDEMDNKMTSCSYVAHER
  LSYAFSVWRMEGAWSMSASH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-Myc/DDK
• Predicted MW: 17.9 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Note: For culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_476527
• Locus ID: 117581
• UniProt ID: Q8WVJ9, A1MB48
• Cytogenetics: 2q37.3
• Refseq Size: 1186
• Refseq ORF: 480
• Synonyms: AMS; BBRSAY; bHLHa39; DERMO1; FFDD3; SETLSS
• Summary: The protein encoded by this gene is a basic helix-loop-helix type transcription factor and shares similarity with Twist. This protein may inhibit osteoblast maturation and maintain cells in a preosteoblast phenotype during osteoblast development. This gene may be upregulated in certain cancers. Mutations in this gene cause focal facial dermal dysplasia 3, Setleis type. Two transcript variants encoding the same protein have been found. [provided by RefSeq, Apr 2014]
• Protein Families: Druggable Genome, Transcription Factors