Ribonuclease Inhibitor (RNH1) (NM_002939) Human Recombinant Protein

CAT#: TP308360

Recombinant protein of human ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 1

Product Images

Inhibition of angiogenin by ribonuclease/angiogenin inhibitor 1 (RNH1) results in a reduction in the ability of SDF-1mig cell-conditioned medium to stimulate the formation of stress granules. Images show the eIF3 granules in cardiomyocytes exposed to the SDF-1mig BM-MNC-conditioned medium in the absence (i) or presence (ii) of RNH1 (OriGene TP308360). Myocyte cytoplasm was labeled with alpha sarcomeric actinin (iii). DAPI stained the nuclei. Figure cited from Stem Cell Res Ther, PMID: 25889213

• 

  Coomassie blue staining of purified RNH1 protein (Cat# TP308360). The protein was produced from HEK293T cells transfected with RNH1 cDNA clone (Cat# RC208360) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >RC208360 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSLDIQSLDIQCEELSDARWAELLPLLQQCQVVRLDDCGLTEARCKDISSALRVNPALAELNLRSNELGD
  VGVHCVLQGLQTPSCKIQKLSLQNCCLTGAGCGVLSSTLRTLPTLQELHLSDNLLGDAGLQLLCEGLLDP
  QCRLEKLQLEYCSLSAASCEPLASVLRAKPDFKELTVSNNDINEAGVRVLCQGLKDSPCQLEALKLESCG
  VTSDNCRDLCGIVASKASLRELALGSNKLGDVGMAELCPGLLHPSSRLRTLWIWECGITAKGCGDLCRVL
  RAKESLKELSLAGNELGDEGARLLCETLLEPGCQLESLWVKSCSFTAACCSHFSSVLAQNRFLLELQISN
  NRLEDAGVRELCQGLGQPGSVLRVLWLADCDVSDSSCSSLAATLLANHSLRELDLSNNCLGDAGILQLVE
  SVRQPGCLLEQLVLYDIYWSEEMEDRLQALEKDKPSLRVIS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-Myc/DDK
• Predicted MW: 49.8 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
• Bioactivity: Cell treatment (PMID: 25889213)
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Note: For culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_002930
• Locus ID: 6050
• UniProt ID: P13489, A0A140VJT8
• Cytogenetics: 11p15.5
• Refseq Size: 2057
• Refseq ORF: 1383
• Synonyms: RAI; RNH
• Summary: Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.[supplied by OMIM, Jul 2010]