NSUN2 (NM_017755) Human Recombinant Protein

CAT#: TP314459

Recombinant protein of human NOL1/NOP2/Sun domain family, member 2 (NSUN2)

Product Images

Coomassie blue staining of purified NSUN2 protein (Cat# TP314459). The protein was produced from HEK293T cells transfected with NSUN2 cDNA clone (Cat# RC214459) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Recombinant protein was produced with TrueORF clone, RC214459. Click on the TrueORF clone link to view cDNA and protein sequences.
• Tag: C-Myc/DDK
• Predicted MW: 86.3 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_060225
• Locus ID: 54888
• UniProt ID: Q08J23
• Cytogenetics: 5p15.31
• Refseq Size: 3089
• Refseq ORF: 2301
• Synonyms: MISU; MRT5; SAKI; TRM4
• Summary: This gene encodes a methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. This modification is necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2011]