PPM1H (NM_020700) Human Recombinant Protein

CAT#: TP317548

Recombinant protein of human protein phosphatase 1H (PP2C domain containing) (PPM1H)

Product Images

Release of free phosphate was measured after incubation of different protein phosphatases, including PPM1H (OriGene TP317548), with the generic phosphopeptide, RRA (pT)VA. * p < 0.01. Figure cited from PLoS ONE, PMID: 25658463

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  Coomassie blue staining of purified PPM1H protein (Cat# TP317548). The protein was produced from HEK293T cells transfected with PPM1H cDNA clone (Cat# RC217548) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Recombinant protein was produced with TrueORF clone, RC217548. Click on the TrueORF clone link to view cDNA and protein sequences.
• Tag: C-Myc/DDK
• Predicted MW: 56.3 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
• Bioactivity: In vitro phosphatase assay (PMID: 25658463)
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_065751
• Locus ID: 57460
• UniProt ID: Q9ULR3
• Cytogenetics: 12q14.1-q14.2
• Refseq Size: 6201
• Refseq ORF: 1542
• Synonyms: ARHCL1; NERPP-2C; URCC2
• Protein Families: Phosphatase