POLK (NM_016218) Human Recombinant Protein

CAT#: TP319806

Recombinant protein of human polymerase (DNA directed) kappa (POLK)

Product Images

Coomassie blue staining of purified POLK protein (Cat# TP319806). The protein was produced from HEK293T cells transfected with POLK cDNA clone (Cat# RC219806) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Recombinant protein was produced with TrueORF clone, RC219806. Click on the TrueORF clone link to view cDNA and protein sequences.
• Tag: C-Myc/DDK
• Predicted MW: 98.6 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_057302
• Locus ID: 51426
• UniProt ID: Q9UBT6, A0A024RAP4
• Cytogenetics: 5q13.3
• Refseq Size: 4074
• Refseq ORF: 2610
• Synonyms: DINB1; DINP; POLQ
• Summary: This gene encodes a member of the DNA polymerase type-Y family of proteins. The encoded protein is a specialized DNA polymerase that catalyzes translesion DNA synthesis, which allows DNA replication in the presence of DNA lesions. Human cell lines lacking a functional copy of this gene exhibit impaired genome integrity and enhanced susceptibility to oxidative damage. Mutations in this gene that impair enzyme activity may be associated with prostate cancer in human patients. [provided by RefSeq, Sep 2016]
• Protein Families: Druggable Genome