CHRDL2 (NM_015424) Human Recombinant Protein

CAT#: TP320245

Recombinant protein of human chordin-like 2 (CHRDL2)

Product Images

CHRDL2 binds to BMP2. Co-IP of recombinant CHRDL2 protein (OriGene TP320245) and recombinant BMP2 protein and analysis in Western blot with indicated antibodies. a: BMP-2 IP with anti-CHRDL2; b: CHRDL2 IP with anti-CHRDL2; c: the mixture of BMP2 and CHRDL2; d: the mixture of BMP2 and CHRDL2 IP with anti-CHRDL2. Figure cited from Oncotarget, PMID: 28009989

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  Coomassie blue staining of purified CHRDL2 protein (Cat# TP320245). The protein was produced from HEK293T cells transfected with CHRDL2 cDNA clone (Cat# RC220245) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Recombinant protein was produced with TrueORF clone, RC220245. Click on the TrueORF clone link to view cDNA and protein sequences.
• Tag: C-Myc/DDK
• Predicted MW: 49.5 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
• Bioactivity: Co-immunoprecipitation (PMID: 28009989)
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_056239
• Locus ID: 25884
• UniProt ID: Q6WN34
• Cytogenetics: 11q13.4
• Refseq Size: 1699
• Refseq ORF: 1353
• Synonyms: BNF1; CHL2
• Summary: This gene encodes a member of the chordin family of proteins. Chordin family members are secreted proteins that share a cysteine-rich pro-collagen repeat domain and associate with members of the transforming growth factor beta superfamily. In vitro assays demonstrate a direct interaction between the encoded protein and human activin A. This gene is expressed in many tissues including osteoblasts, where it is differentially expressed during differentiation. In addition, its expression is upregulated in human osteoarthritic joint cartilage, suggesting a role in adult cartilage regeneration. [provided by RefSeq, Jan 2015]
• Protein Families: Secreted Protein