OGA (NM_012215) Human Recombinant Protein

CAT#: TP322411

Recombinant protein of human meningioma expressed antigen 5 (hyaluronidase) (MGEA5), transcript variant 1

Product Images

Coomassie blue staining of purified OGA protein (Cat# TP322411). The protein was produced from HEK293T cells transfected with OGA cDNA clone (Cat# RC222411) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Recombinant protein was produced with TrueORF clone, RC222411. Click on the TrueORF clone link to view cDNA and protein sequences.
• Tag: C-Myc/DDK
• Predicted MW: 102.7 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_036347
• Locus ID: 10724
• UniProt ID: O60502
• Cytogenetics: 10q24.32
• Refseq Size: 5220
• Refseq ORF: 2748
• Synonyms: MEA5; MGEA5; NCOAT
• Summary: The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]). [supplied by OMIM, Mar 2008]