CYP7B1 (NM_004820) Human Recombinant Protein

CAT#: TP322647

Recombinant protein of human cytochrome P450, family 7, subfamily B, polypeptide 1 (CYP7B1)

Product Images

Coomassie blue staining of purified CYP7B1 protein (Cat# TP322647). The protein was produced from HEK293T cells transfected with CYP7B1 cDNA clone (Cat# RC222647) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Recombinant protein was produced with TrueORF clone, RC222647. Click on the TrueORF clone link to view cDNA and protein sequences.
• Tag: C-Myc/DDK
• Predicted MW: 58.1 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_004811
• Locus ID: 9420
• UniProt ID: O75881, Q05C57
• Cytogenetics: 8q12.3
• Refseq Size: 2395
• Refseq ORF: 1518
• Synonyms: CBAS3; CP7B; SPG5A
• Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis. Mutations in this gene have been associated with hereditary spastic paraplegia (SPG5 or HSP), an autosomal recessive disorder. [provided by RefSeq, Apr 2016]
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Primary bile acid biosynthesis