Aminoadipate aminotransferase (AADAT) (NM_016228) Human Recombinant Protein

CAT#: TP323277

Recombinant protein of human aminoadipate aminotransferase (AADAT), transcript variant 1

Product Images

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  Coomassie blue staining of purified AADAT protein (Cat# TP323277). The protein was produced from HEK293T cells transfected with AADAT cDNA clone (Cat# RC223277) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Recombinant protein was produced with TrueORF clone, RC223277. Click on the TrueORF clone link to view cDNA and protein sequences.
• Tag: C-Myc/DDK
• Predicted MW: 47.2 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
• Bioactivity: The specific activity of KATII was determined by measuring the product Kynurenic acid formation from a conversion of Kynurenine. The reaction was carried out at 37? for 15min in the buffer containing PBS, pH7.4, 2mM a-oxoglutarate, 40µM PLP (pyridoxal 5'-phosphate), and 0.5mM kynurenine as the substrate.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_057312
• Locus ID: 51166
• UniProt ID: Q8N5Z0, Q4W5N8
• Cytogenetics: 4q33
• Refseq Size: 2326
• Refseq ORF: 1275
• Synonyms: KAT2; KATII; KYAT2
• Summary: This gene encodes a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
• Protein Pathways: Lysine biosynthesis, Lysine degradation, Metabolic pathways, Tryptophan metabolism