APE1 (APEX1) (NM_001641) Human Recombinant Protein

CAT#: TP720082

Recombinant protein of human APEX nuclease (multifunctional DNA repair enzyme) 1 (APEX1), transcript variant 1

Product Images

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  Co-immunoprecipitation experiments indicate NaHS, an H2S donor, enhances the interaction between wild-type (WT) EXOG and APE1. Wild-type or mutant (C76A) EXOG was mixed with APE1 (OriGene TP720082) with or without NaHS, and immunoprecipitates were analyzed in Western blot with the indicated antibodies. Figure cited from Sci Rep, PMID: 27808278

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Pro2-Leu318
• Tag: tag free
• Predicted MW: 35.6 kDa
• Concentration: lot specific
• Purity: >95% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: Supplied as a 0.2 um filtered solution of 10mM HEPES, 100mM KCl, 50% Glycerol, pH 7.4.
• Bioactivity: Co-immunoprecipitation (PMID: 27808278)
• Endotoxin: < 0.1 EU per µg protein as determined by LAL test
• Storage: Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
• Stability: Stable for at least 3 months from date of receipt under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001632
• Locus ID: 328
• UniProt ID: P27695, Q5TZP7
• Cytogenetics: 14q11.2
• Synonyms: APE; APE1; APEN; APEX; APX; HAP1; REF1
• Summary: The APEX gene encodes the major AP endonuclease in human cells. It encodes the APEX endonuclease, a DNA repair enzyme with apurinic/apyrimidinic (AP) activity. Such AP activity sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. The AP sites are the most frequent pre-mutagenic lesions that can prevent normal DNA replication. Splice variants have been found for this gene; all encode the same protein. Disruptions in the biological functions related to APEX are associated with many various malignancies and neurodegenerative diseases.[provided by RefSeq, Dec 2019]
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Base excision repair