M-CSF (CSF1) (NM_172212) Human Recombinant Protein

CAT#: TP723739

Purified recombinant protein of Human colony stimulating factor 1 (macrophage) (CSF1), transcript variant 4

Specifications

Product Data

• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Human M-CSF, the region of Glu33-Ser190, from gene Accession# NM_172212.2
• Tag: Tag Free
• Predicted MW: 20.6 kDa
• Concentration: Protein formulated and vialed at >50 ug/mL in specified buffer
• Purity: >98%, as determined by Coomassie stained SDS-PAGE.
• Buffer: 1 x PBS
• Bioactivity: The ED50 is 0.5 - 2 ng/ml, corresponding to a specific activity of 0.5 - 2 x 10^6 units/mg, determined by M-NFS60 cell proliferation induced by human M-CSF in a dose dependent manner.
• Endotoxin: Less than 0.01 ng per µg protein as determined by the LAL method
• Storage: Store at -80°C.
• Stability: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Reference Data

• RefSeq: NP_757351
• Locus ID: 1435
• UniProt ID: P09603, A0A024R0A1
• Cytogenetics: 1p13.3
• Refseq Size: 2772
• Refseq ORF: 1662
• Synonyms: CSF-1; MCSF
• Summary: 'The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]'
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Hematopoietic cell lineage