TNFRSF1A (NM_001065) Human Recombinant Protein

CAT#: TP723870

Purified recombinant protein of Human tumor necrosis factor receptor superfamily, member 1A (sTNF-RI / TNFRSF1A)

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Human sTNF-RI, the region of Ile22-Thr211 with an N-terminal Met, from gene Accession# NP19438.1
• Tag: Tag Free
• Predicted MW: 21.4 kDa
• Concentration: Protein formulated and vialed at >50 ug/mL in specified buffer
• Purity: >95%, as determined by Coomassie stained SDS-PAGE.
• Buffer: 1 x PBS
• Bioactivity: The ED50 is 0.045-0.01 µg/ml, corresponding to a specific activity of 1.0-2.2 x 104 units/mg, determined by a dose-dependent inhibition of 0.25 ng/ml TNF-a induced cytotoxicity in L929 mouse fibroblast cells in the presence of 4 µg/ml actinomycin D.
• Endotoxin: Less than 0.01 ng per µg protein as determined by the LAL method
• Storage: Store at -80°C.
• Stability: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Reference Data

• RefSeq: NP_001056
• Locus ID: 7132
• Cytogenetics: 12p13.31
• Refseq Size: 2236
• Refseq ORF: 1365
• Synonyms: CD120a; FPF; p55; p55-R; p60; TBP1; TNF-R; TNF-R-I; TNF-R55; TNFAR; TNFR1; TNFR55; TNFR60
• Summary: 'This gene encodes a member of the TNF receptor superfamily of proteins. The encoded receptor is found in membrane-bound and soluble forms that interact with membrane-bound and soluble forms, respectively, of its ligand, tumor necrosis factor alpha. Binding of membrane-bound tumor necrosis factor alpha to the membrane-bound receptor induces receptor trimerization and activation, which plays a role in cell survival, apoptosis, and inflammation. Proteolytic processing of the encoded receptor results in release of the soluble form of the receptor, which can interact with free tumor necrosis factor alpha to inhibit inflammation. Mutations in this gene underlie tumor necrosis factor receptor-associated periodic syndrome (TRAPS), characterized by fever, abdominal pain and other features. Mutations in this gene may also be associated with multiple sclerosis in human patients. [provided by RefSeq, Sep 2016]'
• Protein Families: Druggable Genome, Secreted Protein, Transcription Factors, Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Cytokine-cytokine receptor interaction, MAPK signaling pathway