IL6 (NM_000600) Human Recombinant Protein

CAT#: TP750006

Recombinant protein of human Interleukin 6(IL-6) produced in E. coli.

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence encoding the region (Pro29-Met212) of IL6
• Tag: Tag Free
• Predicted MW: 20.9 kDa
• Concentration: Resuspend the protein to the desired concentration in proper buffer.
• Purity: >95% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: Lyophilized from a sterile solution containing 20 mM phosphate buffer, pH7.4.
• Bioactivity: Determined by the dose-dependant stimulation of murine 7TD1 cells. ED50 for this effect is typically 0.009-0.014 ng/ml.
• Endotoxin: < 0.1 EU per 1 µg of the protein by the LAL
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_000591
• Locus ID: 3569
• UniProt ID: P05231, Q75MH2, B4DVM1
• Cytogenetics: 7p15.3
• Refseq Size: 1201
• Refseq ORF: 636
• Synonyms: BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6
• Summary: 'This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). [provided by RefSeq, Aug 2020]'
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Cytosolic DNA-sensing pathway, Graft-versus-host disease, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), Jak-STAT signaling pathway, NOD-like receptor signaling pathway, Pathways in cancer, Prion diseases, Toll-like receptor signaling pathway