BMP2 (NM_001200) Human Recombinant Protein

CAT#: TP750012

Recombinant protein of human Bone Morphogenetic Protein-2 (BMP2) produced in E. coli.

Product Images

• 

  CHRDL2 binds to BMP2. Co-IP of recombinant CHRDL2 protein and recombinant BMP2 protein (OriGene TP750012) and analysis in Western blot with indicated antibodies. a: BMP-2 IP with anti-CHRDL2; b: CHRDL2 IP with anti-CHRDL2; c: the mixture of BMP2 and CHRDL2; d: the mixture of BMP2 and CHRDL2 IP with anti-CHRDL2. Figure cited from Oncotarget, PMID: 28009989

• 

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence encoding the region (Gln283-Arg396) of human BMP2
• Tag: Tag Free
• Predicted MW: 13 kDa
• Concentration: Resuspend the protein to the desired concentration in proper buffer.
• Purity: >95% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: Lyophilized from a sterile solution containing 10mM sodium citrate, pH 3.5.
• Bioactivity: Measured?by?its?ability?to?induce?alkaline?phosphatase?production in?ATDC5?cells. ED50 for this effect is typically 36 - 55 ng/ml.
  Co-immunoprecipitation (PMID: 28009989)
• Endotoxin: < 0.1 EU per 1 µg of the protein by the LAL
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_001191
• Locus ID: 650
• UniProt ID: P12643, C8C060
• Cytogenetics: 20p12.3
• Refseq Size: 1547
• Refseq ORF: 1188
• Synonyms: BDA2; BMP2A; SSFSC
• Summary: 'This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone and cartilage development. Duplication of a regulatory region downstream of this gene causes a form of brachydactyly characterized by a malformed index finger and second toe in human patients. [provided by RefSeq, Jul 2016]'
• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transmembrane
• Protein Pathways: Acute myeloid leukemia, Basal cell carcinoma, Cytokine-cytokine receptor interaction, Endocytosis, Hedgehog signaling pathway, Hematopoietic cell lineage, Melanogenesis, Pathways in cancer, TGF-beta signaling pathway