MIRLET7A1 Human MicroRNA Expression Plasmid (MI0000060)
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Specifications
Product Data | |
Species | Human |
Vector | pCMV-MIR |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
Precursor:
UGGGAUGAGGUAGUAGGUUGUAUAGUUUUAGGGUCACACCCACCACUGGGAGAUAACUAUACAAUCUACUGUCUUUCCUA
Mature Sequence:
UGAGGUAGUAGGUUGUAUAGUU
>MI0000060
GTGGTAAGAGGGTGATTTCTGAATCTTGTAAATACATGGTTTTAGGAGCGGATTCAGATAACCAAGCATT
TAAAATACTATTAATGAAATACAGGAAATGAAACCACAGCATAGATTATGCATGTAGCCAAAATGTTCAG TTAAACTTCATTTTCAACGTAAGTGAATGAAAATGGTCTAATACTATTTTTCTTATCACTCACACAGGAA ACCAGGATTACCGAGGAGGAAAAAAAGCCTTCCTGTGGTGCTCAACTGTGATTCCTTTTCACCATTCACC CTGGATGTTCTCTTCACTGTGGGATGAGGTAGTAGGTTGTATAGTTTTAGGGTCACACCCACCACTGGGA GATAACTATACAATCTACTGTCTTTCCTAACGTGATAGAAAAGTCTGCATCCAGGCGGTCTGATAGAAAG TCAGTTAACTAATTGTACAATATTTAAGATTAACTTGTCTTAAAGAGATGTAGTGCAGCATTTGTTTATG GCCTGGAAATAAATTAATTTAGAGATAAAGTCTGTAGCAAGTACACTGGATGGGGGTGGGGAAACCTTTT GCTTCTTGTCTTATTTCTCTGTGTCAGAATAAATGTATTTTTTTATTTTGATTTATGCTGATAATTTTAT GTTGAAATTTTCTTTCGAAAGAGATTGTACTTTCCATTCCAGAAG |
OTI Disclaimer | All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA. |
Product Components | The plasmid is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. |
Also Contains | Both mature and minor (star) miRNA can be over expressed from the construct. |
Reference Data | |
RefSeq | MI0000060 |
Synonyms | LET7A1 let-7a-1 MIRNLET7A1 |
Locus ID | 406881 |
Documents
Product Manuals |
FAQs |
SDS |
Resources
RNAi Resources |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
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in shipping.