MIRLET7d Human MicroRNA Expression Plasmid (MI0000065)

CAT#: SC400006

Expression plasmid for human microRNA LET7d

Specifications

Product Data

• Species: Human
• Vector: pCMV-MIR
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Precursor:

  CCUAGGAAGAGGUAGUAGGUUGCAUAGUUUUAGGGCAGGGAUUUUGCCCACAAGGAGGUAACUAUACGACCUGCUGCCUUUCUUAGG
  Mature Sequence:

  AGAGGUAGUAGGUUGCAUAGUU
  Full insert

  >MI0000065 GCGACTCCAGTAATACGTGGTGGGCTGAGTATTAGACTACTGGCTTACATCCTCTGATGTCATTACTTTC
  TTGTTCGTTTGTGACTTGAGGTTGGATTTTAAGCAAATGTATTTGTGGCTTTTTACCAAGGTCATATGGC
  CAGATAACTTTTCAAAAGCATTAGTTAAAGAATTCTGATTAGTTTGAATTAGAAACAAAACTCAAAGAAC
  ATGACCTAATTTAACAGGTTAATTTGAAGTGCATCTGCCAAGTAGAAGACCAGCAAGAAAAAAAAAATGG
  GTTCCTAGGAAGAGGTAGTAGGTTGCATAGTTTTAGGGCAGGGATTTTGCCCACAAGGAGGTAACTATAC
  GACCTGCTGCCTTTCTTAGGGCCTTATTATTCACCGATAACCTGTTTCCTTGCTACTTTGCTTTGGTGTA
  AGCAGAGTTCTTTCTGTAGGTTTTTTCAAATGAAAACATTGCAAGAATATCAAAGAGAGCAGTGTTTGCG
  TTAGTGATTATAAACTGCAGCATGGTGCTGACATTGATAACTGAAAGTCAACTAATGAGAATTTGAGACT
  TCTGAAGTACACTTAGTTGCTAGTGTCTCCCTTTTGGTGTCACTGGAAAGTTTAGAAAGCATGGTTTTGT
  TTTTGCTCAGGTTTCTCTTTCTGTGATGC
• OTI Disclaimer: All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
• Product Components: The plasmid is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Also Contains: Both mature and minor (star) miRNA can be over expressed from the construct.

Reference Data

• RefSeq: MI0000065
• Synonyms: LET7D let-7d MIRNLET7D hsa-let-7d
• Locus ID: 406886