MIRLET7f-1 Human MicroRNA Expression Plasmid (MI0000067)

CAT#: SC400008

Expression plasmid for human microRNA LET7f-1

Specifications

Product Data

• Species: Human
• Vector: pCMV-MIR
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Precursor:

  UCAGAGUGAGGUAGUAGAUUGUAUAGUUGUGGGGUAGUGAUUUUACCCUGUUCAGGAGAUAACUAUACAAUCUAUUGCCUUCCCUGA
  Mature Sequence:

  UGAGGUAGUAGAUUGUAUAGUU
  Full insert

  >MI0000067 TCTGCATCCAGGCGGTCTGATAGAAAGTCAGTTAACTAATTGTACAATATTTAAGATTAACTTGTCTTAA
  AGAGATGTAGTGCAGCATTTGTTTATGGCCTGGAAATAAATTAATTTAGAGATAAAGTCTGTAGCAAGTA
  CACTGGATGGGGGTGGGGAAACCTTTTGCTTCTTGTCTTATTTCTCTGTGTCAGAATAAATGTATTTTTT
  TATTTTGATTTATGCTGATAATTTTATGTTGAAATTTTCTTTCGAAAGAGATTGTACTTTCCATTCCAGA
  AGAAAACATTGCTCTATCAGAGTGAGGTAGTAGATTGTATAGTTGTGGGGTAGTGATTTTACCCTGTTCA
  GGAGATAACTATACAATCTATTGCCTTCCCTGAGGAGTAGACTTGCTGCATTATTTTCTTTTTATTTAGA
  TGATATTAAAACTCAGAAGAATTAATTTTGACATTTTGTATTTACAGTTTATCAGTTAATTTTCTCTGTT
  CAAGTAGTACAGTAGGCACAGATTAACATTTAAATTTTTCACATATGGTATATTTCAGAAATTTGAAGTT
  AAGCAAAAATTTTAATGAGTAGAGAAAGTAAGTAGCCTTCAGGAAATCTTCATAGAGGACCAGGCCCTTT
  TGGAATTGTGAATAGGTTTATTGCCTTACATCCTGGTACACATGTCCAAGG
• OTI Disclaimer: All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
• Product Components: The plasmid is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Also Contains: Both mature and minor (star) miRNA can be over expressed from the construct.

Reference Data

• RefSeq: MI0000067
• Synonyms: LET7F1 let-7f-1 MIRNLET7F1
• Locus ID: 406888