MIR16-1 Human MicroRNA Expression Plasmid (MI0000070)

CAT#: SC400199

Expression plasmid for human microRNA MIR16-1

Specifications

Product Data

• Species: Human
• Vector: pCMV-MIR
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Precursor:

  GUCAGCAGUGCCUUAGCAGCACGUAAAUAUUGGCGUUAAGAUUCUAAAAUUAUCUCCAGUAUUAACUGUGCUGCUGAAGUAAGGUUGAC
  Mature Sequence:

  UAGCAGCACGUAAAUAUUGGCG
  Full insert

  >MI0000070 ATCTCCTTAAAAATAATTATGCATATTACATCAATGTTATAATGTTTAAACATAGATTTTTTTACATGCA
  TTCTTTTTTTCCTGAAAGAAAATATTTTTTATATTCTTTAGGCGCGAATGTGTGTTTAAAAAAAATAAAA
  CCTTGGAGTAAAGTAGCAGCACATAATGGTTTGTGGATTTTGAAAAGGTGCAGGCCATATTGTGCTGCCT
  CAAAAATACAAGGATCTGATCTTCTGAAGAAAATATATTTCTTTTTATTCATAGCTCTTATGATAGCAAT
  GTCAGCAGTGCCTTAGCAGCACGTAAATATTGGCGTTAAGATTCTAAAATTATCTCCAGTATTAACTGTG
  CTGCTGAAGTAAGGTTGACCATACTCTACAGTTGTGTTTTAATGTATATTAATGTTACTAATGTGTTTTC
  AGTTTTATTGATAGTCTTTTCAGTATTATTGATAATCTTGTTATTTTTAGTATGATTCTGTAAAAATGAA
  TTAATACTAATTTTTCAGATGTATCATCTCTTAAAATACTGTAATTGCAATTTAATAATTGTATTGAATG
  CCATCAAGTTTTTTTAAAAAGCTTATGCAGCATTAGAGGAATTTATTTTAATGCACATTTATATTCAACA
  TAGACATTAATTCAGATTTTTACTTGGGA
• OTI Disclaimer: All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
• Product Components:
  • 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
  • 1 x 100 pmol VP1.5 primer
  • 1 x 100 pmol XL39 primer
• Also Contains: Both mature and minor (star) miRNA can be over expressed from the construct.

Reference Data

• RefSeq: MI0000070
• Synonyms: MIRN16-1 miRNA16-1
• Locus ID: 406950