MIR20a Human MicroRNA Expression Plasmid (MI0000076)

CAT#: SC400269

Expression plasmid for human microRNA MIR20a

Specifications

Product Data

• Species: Human
• Vector: pCMV-MIR
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Precursor:

  GUAGCACUAAAGUGCUUAUAGUGCAGGUAGUGUUUAGUUAUCUACUGCAUUAUGAGCACUUAAAGUACUGC
  Mature Sequence:

  UAAAGUGCUUAUAGUGCAGGUAG
  Full insert

  >MI0000076 GCATCTACTGCCCTAAGTGCTCCTTCTGGCATAAGAAGTTATGTATTCATCCAATAATTCAAGCCAAGCA
  AGTATATAGGTGTTTTAATAGTTTTTGTTTGCAGTCCTCTGTTAGTTTTGCATAGTTGCACTACAAGAAG
  AATGTAGTTGTGCAAATCTATGCAAAACTGATGGTGGCCTGCTATTTCCTTCAAATGAATGATTTTTACT
  AATTTTGTGTACTTTTATTGTGTCGATGTAGAATCTGCCTGGTCTATCTGATGTGACAGCTTCTGTAGCA
  CTAAAGTGCTTATAGTGCAGGTAGTGTTTAGTTATCTACTGCATTATGAGCACTTAAAGTACTGCTAGCT
  GTAGAACTCCAGCTTCGGCCTGTCGCCCAATCAAACTGTCCTGTTACTGAACACTGTTCTATGGTTAGTT
  TTGCAGGTTTGCATCCAGCTGTGTGATATTCTGCTGTGCAAATCCATGCAAAACTGACTGTGGTAGTGAA
  AAGTCTGTAGAAAAGTAAGGGAAACTCAAACCCCTTTCTACACAGGTTGGGATCGGTTGCAATGCTGTGT
  TTCTGTATGGTATTGCACTTGTCCCGGCCTGTTGAGTTTGGTGGGGATTGTGACCAGAAGAT
• OTI Disclaimer: All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
• Product Components:
  • 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
  • 1 x 100 pmol VP1.5 primer
  • 1 x 100 pmol XL39 primer
• Also Contains: MI0000074
  Both mature and minor (star) miRNA can be over expressed from the construct.

Reference Data

• RefSeq: MI0000076
• Synonyms: MIR20 MIRN20 miR-20 MIRN20A miRNA20A hsa-mir-20 hsa-mir-20a
• Locus ID: 406982