MIR21 Human MicroRNA Expression Plasmid (MI0000077)

CAT#: SC400271

Expression plasmid for human microRNA MIR21

Specifications

Product Data

• Species: Human
• Vector: pCMV-MIR
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Precursor:

  UGUCGGGUAGCUUAUCAGACUGAUGUUGACUGUUGAAUCUCAUGGCAACACCAGUCGAUGGGCUGUCUGACA
  Mature Sequence:

  UAGCUUAUCAGACUGAUGUUGA
  Full insert

  >MI0000077 CAGTTTTCTTGCCGTTCTGTAAGTGTTTTATTCTTAGTGTGATTTTTTTCCATTGGGATGTTTTTGATTG
  AACTTGTTCATTTTGTTTTGCTTGGGAGGAAAATAAACAATTTTACTTTTTTCCTTTAGGAGCATTATGA
  GCATTATGTCAGAATAGAATAGAATTGGGGTTCGATCTTAACAGGCCAGAAATGCCTGGGTTTTTTTGGT
  TTGTTTTTGTTTTTGTTTTTTTATCAAATCCTGCCTGACTGTCTGCTTGTTTTGCCTACCATCGTGACAT
  CTCCATGGCTGTACCACCTTGTCGGGTAGCTTATCAGACTGATGTTGACTGTTGAATCTCATGGCAACAC
  CAGTCGATGGGCTGTCTGACATTTTGGTATCTTTCATCTGACCATCCATATCCAATGTTCTCATTTAAAC
  ATTACCCAGCATCATTGTTTATAATCAGAAACTCTGGTCCTTCTGTCTGGTGGCACTTAGAGTCTTTTGT
  GCCATAATGCAGCAGTATGGAGGGAGGATTTTATGGAGAAATGGGGATAGTCTTCATGACCACAAATAAA
  TAAAGGAAAACTAAGCTGCATTGTGGGTTTTGAAAAGGTTATTATACTTCTTAACAATTCTTTTTTTCAG
  GGACTTTTCTAGCTGTATGAC
• OTI Disclaimer: All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
• Product Components:
  • 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
  • 1 x 100 pmol VP1.5 primer
  • 1 x 100 pmol XL39 primer
• Also Contains: Both mature and minor (star) miRNA can be over expressed from the construct.

Reference Data

• RefSeq: MI0000077
• Synonyms: MIRN21 miR-21 miRNA21 hsa-mir-21
• Locus ID: 406991