MIR325 Human MicroRNA Expression Plasmid (MI0000824)

CAT#: SC400339

Expression plasmid for human microRNA MIR325

Specifications

Product Data

• Species: Human
• Vector: pCMV-MIR
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Precursor:

  AUACAGUGCUUGGUUCCUAGUAGGUGUCCAGUAAGUGUUUGUGACAUAAUUUGUUUAUUGAGGACCUCCUAUCAAUCAAGCACUGUGCUAGGCUCUGG
  Mature Sequence:

  CCUAGUAGGUGUCCAGUAAGUGU
  Full insert

  >MI0000824 AGCCTGAAAAGCTCTCTGTAAGGAACCAGATTTCAGAACAGGCCTCTCTCTGGCCATTTCCTACAGCAAC
  TCTTCACAATTAGTCCTTTTGGGTCTGCTGAGTTCTATCAAGCAATCATTCAACAAATGTTGAATGACCA
  TTATGAGTAAAGTCACGGCACGGGACAGGGATTGTATGGCTTAATTAAATATACTGACTTGACATTCACA
  AAGGAAAGAAAGGTTCTGTGAGAAAAAGTTGCTTAAATATAGGTTTTGAGATGGATTCAAGTCCACAGAA
  CCAATACAGTGCTTGGTTCCTAGTAGGTGTCCAGTAAGTGTTTGTGACATAATTTGTTTATTGAGGACCT
  CCTATCAATCAAGCACTGTGCTAGGCTCTGGGACTACATAGATAAGTTATTCAACATCCTACATTTTGAG
  GTACTTAGGCCTAGTGGTGGTTACAGTAGAGTGATTAATATTTTGACAAAGGTACAAACGGGTAGGAGAG
  GATCTACCCCAGAAACAGAACCAGATTTCAGTGTGTTGAGGGGTGAAAAGGAAAAGAGTAAGTGAAGATG
  GTGAATGTAAATGGTTCTTTAAAAGTTTTTAAACTGTTCTTAAACTGCTTTTAAAAAGCTTGGTTAGAAA
  GAAAAGGAGAGACAGGGCAACAAACAG
• OTI Disclaimer: All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
• Product Components:
  • 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
  • 1 x 100 pmol VP1.5 primer
  • 1 x 100 pmol XL39 primer
• Also Contains: Both mature and minor (star) miRNA can be over expressed from the construct.

Reference Data

• RefSeq: MI0000824
• Synonyms: MIRN325 hsa-mir-325
• Locus ID: 442899