MIR330 Human MicroRNA Expression Plasmid (MI0000803)

CAT#: SC400344

Expression plasmid for human microRNA MIR330

Specifications

Product Data

• Species: Human
• Vector: pCMV-MIR
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Precursor:

  CUUUGGCGAUCACUGCCUCUCUGGGCCUGUGUCUUAGGCUCUGCAAGAUCAACCGAGCAAAGCACACGGCCUGCAGAGAGGCAGCGCUCUGCCC
  Mature Sequence:

  UCUCUGGGCCUGUGUCUUAGGC
  Full insert

  >MI0000803 CGCCATGAGTAGCTTTGGAGCTGGGTGAGACCCTTTCGCAGACCACCCCCCATCTCCTCTCCGCGCTCCC
  GGGGCTTAGATCTCAGGTTTCACCCAACCCCTGGGACCCAGACCGGCGTGGGGACACGCCCCTTCCCTTA
  AACTCTCCCCGTTTCTCCCTCTGCTTGACGTTTGGTGTGCTGGGGGAACTGCGGGTGGGGGGCGCTGGGG
  AGCACCTTGCTGATTAGGAGGGAAGGGTCCTTGGTGACTCCCTTCTTCCAGGATCGCGTCCCTGCCACTT
  CGTGCTGTGTGATCTTTGGCGATCACTGCCTCTCTGGGCCTGTGTCTTAGGCTCTGCAAGATCAACCGAG
  CAAAGCACACGGCCTGCAGAGAGGCAGCGCTCTGCCCCTTACTCGGCCCCGTTTTCATCGGAGACCTCCG
  GGGAGCGGTGGGGGTGGAGGAATGGTTTCTCCCCTTTTCTGAACTGAATACTAAGACCCTTTTTTTTTCT
  TTGTCCTTTCCTGACAGCAAAACCAAAGAAGTTATCTTCAGTGTGGGTGAGTGGGGAGATGGGGAAGGGC
  TCGGTGGAAGCTTGCTTGTTGGGGTGACAGGCTGGAGCCAGAGGTCAGGAGTCTTGGCTACTGGGTCTTT
  GCCTCTCTGGCCTCAGTTTCCCTGCCTTTAA
• OTI Disclaimer: All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
• Product Components:
  • 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
  • 1 x 100 pmol VP1.5 primer
  • 1 x 100 pmol XL39 primer
• Also Contains: Both mature and minor (star) miRNA can be over expressed from the construct.

Reference Data

• RefSeq: MI0000803
• Synonyms: MIRN330 hsa-mir-330
• Locus ID: 442902