MIR653 Human MicroRNA Expression Plasmid (MI0003674)

CAT#: SC400630

Expression plasmid for human microRNA MIR653

Specifications

Product Data

• Species: Human
• Vector: pCMV-MIR
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Precursor:

  UUCAUUCCUUCAGUGUUGAAACAAUCUCUACUGAACCAGCUUCAAACAAGUUCACUGGAGUUUGUUUCAAUAUUGCAAGAAUGAUAAGAUGGAAGC
  Mature Sequence:

  GUGUUGAAACAAUCUCUACUG
  Full insert

  >MI0003674 GCATCCTCAGATTTTGGTATCCACATATGCAAATACTACACCATTTTATATGAAGTACCTAAGCATCCTC
  AGATTTTGGTATCCACAGGAGGTCCTGCAACCAATCCCCTGAGTATACCAAGGGATGACTGTATGTATAA
  ACGTAATGCCCCAATGGGTATGTTATTTTAGGAAGAAGGCCACTGAGACATTCATCTACTGTTGGGAAAA
  TTCCATCTTCAGTGCTCAAATATGAACCAGACCAGGAACTGATGCAGATTATTACTGCTCATCTGACAAC
  AGAGGTTCTAATTTTTCATTCCTTCAGTGTTGAAACAATCTCTACTGAACCAGCTTCAAACAAGTTCACT
  GGAGTTTGTTTCAATATTGCAAGAATGATAAGATGGAAGCTACTTTCATCAGCATGAATTATAACTACTC
  GTATACAGATTGTCGTATTTCCACTGTTACTGATGTTAAAACTGCATTAAAGCAAATAAAAATATAATTG
  AAAATACAAGCATAGGGTTTTCTACTTGTTATCCTCGGACATACTATTTGCCTATGGTTTCCATGAATTT
  CCAACTAGAGCAAGAAAATCTCTTGGTTGGTTGTCAGCCATATAAAAAGAGAATTCAGAATGTTACTTTT
  GGGAAGAAAGCCAGATTTAAACAAAATACTTCCCAGTGTTTAGCATA
• OTI Disclaimer: All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
• Product Components:
  • 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
  • 1 x 100 pmol VP1.5 primer
  • 1 x 100 pmol XL39 primer
• Also Contains: Both mature and minor (star) miRNA can be over expressed from the construct.

Reference Data

• RefSeq: MI0003674