Mir218-1 Mouse MicroRNA Expression Plasmid (MI0000700)

CAT#: SC400924

Expression plasmid for mouse microRNA Mir218-1

Specifications

Product Data

• Species: Mouse
• Vector: pCMV-MIR
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Precursor:

  GUGAUAAUGGAGCGAGAUUUUCUGUUGUGCUUGAUCUAACCAUGUGCUUGCGAGGUAUGAGAAAAACAUGGUUCCGUCAAGCACCAUGGAACGUCACGCAGCUUUCUACA
  Mature Sequence:

  UUGUGCUUGAUCUAACCAUGU
  Full insert

  >MI0000700 GAGCTTCTCTGTTGTCCTTGTAGCTTACCTTCAACTTCTTCCTGGATGTTCTTCCCGAAAGCTGTGTTTC
  CCACGCTGCAGTTGTGAGGAACACGGGAGGCGCCTTTCCATCTAGCCCCTTCTTTGTGTTTCCAGGCACC
  TTCGGTAGTTATGCTCTCGCTATGATCATACACAATCTGCGGGAAGAATGCATGTCATGTAACATTAGGA
  TGCTTCCAGAGCGTCCGTAGCTTCTCTCCTCCCTTCGTCTTTCCTCTCGGTCTTCCCTCTGGCCTAGTGG
  TTGGTGTAGTGATAATGGAGCGAGATTTTCTGTTGTGCTTGATCTAACCATGTGCTTGCGAGGTATGAGA
  AAAACATGGTTCCGTCAAGCACCATGGAACGTCACGCAGCTTTCTACAGCATGGCAAGCTGCTGAGGCTC
  GAGGCAGGTCTTACTTGTCTTTGTTTTTTTTTTTTTTAATAGACTCAAAATGAACATAAAAGAGGGCTTT
  GGGGGCATTTCTGAAGATTATGTGAGAGGGGAGAATAACAAATGTCCGTAGGAAAAAAAAATTAAAACCT
  AAGTATATCGATTATCCTTGAAAAGCCCTACTTTACAATAATATACAGGAGTATGTTTATTATGAGATAT
  GTTTTCAATTTTTGACTCATAAAAATTTTGACTCATAAAATTAAACTTTTTTTAACAAT
• OTI Disclaimer: All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
• Product Components:
  • 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
  • 1 x 100 pmol VP1.5 primer
  • 1 x 100 pmol XL39 primer
• Also Contains: Both mature and minor (star) miRNA can be over expressed from the construct.

Reference Data

• RefSeq: MI0000700
• Synonyms: Mirn218-1 mir-218-1
• Locus ID: 723822