Mir221 Mouse MicroRNA Expression Plasmid (MI0000709)

CAT#: SC400930

Expression plasmid for mouse microRNA Mir221

Specifications

Product Data

• Species: Mouse
• Vector: pCMV-MIR
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Precursor:

  AUCCAGGUCUGGGGCAUGAACCUGGCAUACAAUGUAGAUUUCUGUGUUUGUUAGGCAACAGCUACAUUGUCUGCUGGGUUUCAGGCUACCUGGAA
  Mature Sequence:

  AGCUACAUUGUCUGCUGGGUUUC
  Full insert

  >MI0000709 CTTGTCTTCCTCTTTCCCCCATGAAGATATCATTTTCTATCCAGCTGTCTATGTATCATCTTCCTAAATG
  ATATCTATCATGTCCCTATCTGTACTTCCATACATCTCTTGTCCTTTAATAGTCATTTTAAAAATAGCAT
  GATGAATGACCACCAATCGAATTATTTGAGGAAATTTCACCTATTGGTAAGTCACAGGAATTGTGACTTC
  CATTTTCTTTTCTTATGGTAAATAAGACACATACCAACTTTGACATGTTAATCTGTATGGATAATTTGCA
  GGCTGAACATCCAGGTCTGGGGCATGAACCTGGCATACAATGTAGATTTCTGTGTTTGTTAGGCAACAGC
  TACATTGTCTGCTGGGTTTCAGGCTACCTGGAATCATTTCTTCATTGGCCTACTTATCATTGTTTTATCT
  CTAACATTTCTGAGTCAACTTTTCTAGGAGCCATAAATGAGTATGACTTATCTCATTGAAAAGAACAAAA
  GAAGGACCTCTATGAAGCTGAGCATCATAGCAAATAATGGTCTCACTGTGATTTCATCCAAAACAGTCTC
  ACATGCCAGACTTGTCAACTGAAGAAACAGAAGAGAAGACAGGTCATATTATTATAAAATGTTTGGGTTT
  GCTATTTGTTATTGCTGACAGGAAGTAAATCATCTTAGCATACTGCTTCCGA
• OTI Disclaimer: All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
• Product Components:
  • 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
  • 1 x 100 pmol VP1.5 primer
  • 1 x 100 pmol XL39 primer
• Also Contains: Both mature and minor (star) miRNA can be over expressed from the construct.

Reference Data

• RefSeq: MI0000709
• Synonyms: Mirn221 mmu-mir-221
• Locus ID: 723827