Mir25 Mouse MicroRNA Expression Plasmid (MI0000689)

CAT#: SC400938

Expression plasmid for mouse microRNA Mir25

Specifications

Product Data

• Species: Mouse
• Vector: pCMV-MIR
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Precursor:

  GGCCAGUGUUGAGAGGCGGAGACUUGGGCAAUUGCUGGACGCUGCCCUGGGCAUUGCACUUGUCUCGGUCUGACAGUGCCGGCC
  Mature Sequence:

  CAUUGCACUUGUCUCGGUCUGA
  Full insert

  >MI0000689 GCTGCTTGAATCCATGAGGGCTAGGACTTGAATTCCTGGTGTCCTAGATATGATCTTAGAAATCTCCATT
  CTGTCCTCCCCTCTTGGACCTTAGTCATGGGGGCTCCAAAGTGCTGTTCGTGCAGGTAGTGTAATTACCT
  GACCTACTGCTGAGCTAGCACTTCCCGAGCCCCCAGGACACAACCTCTGATAGTGGCCCTAGCTGAGCGC
  CCCAGGCTCCATTTGTTTGATGGGGAGCCTAGTGGAGTTCAGAAGGGTCTGGTCTCCCTCACAGGACAGC
  TGAACACCCACCGGGACTGGCCAGTGTTGAGAGGCGGAGACTTGGGCAATTGCTGGACGCTGCCCTGGGC
  ATTGCACTTGTCTCGGTCTGACAGTGCCGGCCCAACACTGCAGATGTGGGGGGGCAGGGAGGAGTCCCCA
  TTTTTGTTCAATGAGTAGGCAAATGTGGCTTGCCCAGGCTTGAAGCTGGAGCAAAGAAAGATCCTTGACT
  TCATTCCCCTCCTCCCCTCCTATCCCAATCTTTTCTATGCCTTCCCCTTAGGCTCGACTTCGGATGGTAG
  ACATTGTGGAGAAGGAAGATGTAAATGAAGCCATAAGGTTGATGGAGATGTCGAAGGACTCCCTTCTAGG
  TGAAAAGGGACAGACAGCTAGGTGAGGAT
• OTI Disclaimer: All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
• Product Components:
  • 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
  • 1 x 100 pmol VP1.5 primer
  • 1 x 100 pmol XL39 primer
• Also Contains: MI0000581, MI0000689
  Both mature and minor (star) miRNA can be over expressed from the construct.

Reference Data

• RefSeq: MI0000689
• Synonyms: Mirn25 mmu-mir-25
• Locus ID: 723926