MIR124-1 Rat MicroRNA Expression Plasmid (MI0000893)

CAT#: SC403123

Expression Plasmid of Rat microRNA MIR124-1

Specifications

Product Data

• Species: Rat
• Vector: pCMV-MIR
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Precursor:

  AGGCCUCUCUCUCCGUGUUCACAGCGGACCUUGAUUUAAAUGUCCAUACAAUUAAGGCACGCGGUGAAUGCCAAGAAUGGGGCUG
  Mature Sequence:

  CGUGUUCACAGCGGACCUUGAU
  Full insert

  >MI0000893 GGGGAGCTGCGGTAGGAAGGATGCGGTGGTCCTTAGCTCATCGCGTTCCCCAAACCCCACACCCTCTCTG
  CTGTCAGTGCGCACGCACACGCACGCGTCGCCAGCTCTTTTTTTTTCTCATCCCTCGTTTTTTTCATTTC
  ATCTTCTCCCCATCCCCTCCCTTTCTTTCCACCTTTCCTTCCTTTCTCCCTCCCTCCTTTTCTTCCCCCT
  CCCCCCTCCCTTTCTCCTATCCTCCCTCCCTCCCATCCTTCCTTCTTCCGATCTTTCTTTCCTTCCTTCC
  TTCTTCCTTCCTCAGGAGAAAGGCCTCTCTCTCCGTGTTCACAGCGGACCTTGATTTAAATGTCCATACA
  ATTAAGGCACGCGGTGAATGCCAAGAATGGGGCTGTCTGAGCACCTTGGGTCCACGAGGGCCCGCCACGG
  AAGGATCGACTCAGCACAACGCCTTGTGCAGATTTCAGTGCTGCAGCTGCAGGCGCCCACAGTAGGGGTA
  GCGGACTTCTAAGTGCCTCGCAATGTAGCCCTTAGGGTGGGGAGGTGTGCGGCTACCAACAGAGAGGGGT
  TTGTGCAGATGCGAATAGAATCTAGGAAGCAAACAACTCGTTCTGTGATAGGAACACGTATCTATGCAAG
  CAGGGTGGTGGTGAAAGTAGAAAATAGCCTGGCAGGAGGGTGGAGAGTGAAACGT
• OTI Disclaimer: All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
• Product Components:
  • 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
  • 1 x 100 pmol VP1.5 primer
  • 1 x 100 pmol XL39 primer
• Also Contains: Both mature and minor (star) miRNA can be over expressed from the construct.

Reference Data

• RefSeq: MI0000893
• Synonyms: rno-mir-124-1
• Locus ID: 100314291