MIR205 Rat MicroRNA Expression Plasmid (MI0000947)

CAT#: SC403212

Expression Plasmid of Rat microRNA MIR205

Specifications

Product Data

• Species: Rat
• Vector: pCMV-MIR
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Precursor:

  AAACAGCCCCAGACAAUCCAUGGGUCCUCCUGUCCUUCAUUCCACCGGAGUCUGUCUUAUGCCAACCAGAUUUCAGUGGAGUGAAGCUCAGGAGGCAUGGAGCUGCCA
  Mature Sequence:

  UCCUUCAUUCCACCGGAGUCUGU
  Full insert

  >MI0000947 TATCCCCAAACATACCCAGCTCTGGTATGGAACACACGTTGAAGAGGAGAGCCAGCATTCCAACTAGGTA
  TACAGAGAGGAGACTCTACTTGGATATATTTGGGATGGCCCTTCATGGGCAAGAAACTACCTAGAGTCCA
  GCCCCACAACATTGGCTGTACCACCCAGAACGCTCTGGCTCTGAGGTGGGAGCATAGATACTTGAGGGTT
  TGAGTATTCAGGTCTTATAAGAGTGAGGTTGCAGGATCCCGAAGGTCTCCTCCAGGTTACACAGCACTAC
  AAGAGGAGGCTTAATAGACAAAACAGCCCCAGACAATCCATGGGTCCTCCTGTCCTTCATTCCACCGGAG
  TCTGTCTTATGCCAACCAGATTTCAGTGGAGTGAAGCTCAGGAGGCATGGAGCTGCCAGCGGTGGACTGA
  AGCACAGCATCTGAACAGAGGCATTCTGCAAAAATACAATCACCAAAAGCATCCGAGGCGGCATGTGCTT
  TTGCGGGGGGCCGGGAGGGGGGATGGCAATGACAGACAAGAGCATACTGCCAATGATTGCTGTGTGGCCT
  TTCTTCCTAAAACCAGTGTTCCAGGAATGTGTAGTTTCTGTAACTTGTGAAATTAAAAGTTGCCCATCTG
  CTGACAAGCCTTCCTGCCTCTGTGCCCTATGCTATCAGCATCCTCAACCTGTCCCTGGGGGCTTAAATCC
  CAGATAGG
• OTI Disclaimer: All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
• Product Components:
  • 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
  • 1 x 100 pmol VP1.5 primer
  • 1 x 100 pmol XL39 primer
• Also Contains: Both mature and minor (star) miRNA can be over expressed from the construct.

Reference Data

• RefSeq: MI0000947
• Synonyms: rno-mir-205
• Locus ID: 100314246