MIR214 Rat MicroRNA Expression Plasmid (MI0000954)

CAT#: SC403223

Expression Plasmid of Rat microRNA MIR214

Specifications

Product Data

• Species: Rat
• Vector: pCMV-MIR
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Precursor:

  GUCCUGGAUGGACAGAGUUGUCAUGUGUCUGCCUGUCUACACUUGCUGUGCAGAACAUCCGCUCACCUGUACAGCAGGCACAGACAGGCAGUCACAUGACAACCCAGCCU
  Mature Sequence:

  AGAGUUGUCAUGUGUCU
  Full insert

  >MI0000954 GGGCCAGTAACGATAGGAGTTGGATTTTTAAAAAAAATTATGCTGTTTTTTTTTCCTTAAAATCGTGTGA
  ATTTTCTGTTTTTGACAACCATCCTTGTTAGCAACATTCTGATCACGTGTATGAGTGTACTTCCTGAATA
  AAGTCAAACGTCTAACTTCTTTTTAGTTCCATAATGTTTAATGTTTAATTCTATTGTGTGTTTTTCTCCT
  TTCCCTTTATCCCCTCTCCTTCAATCACCAAATCTGGAAAACAGGCTGATTGTATCTGTCCAAGAAAAAA
  GGAACCGCGAAGGAGCCATGGTCCTGGATGGACAGAGTTGTCATGTGTCTGCCTGTCTACACTTGCTGTG
  CAGAACATCCGCTCACCTGTACAGCAGGCACAGACAGGCAGTCACATGACAACCCAGCCTGAATGACAAC
  CAGCCATTGAAAGAAAGCTGCCCTCACACCATAGCATCTACACCAAGAGCTACAACCACAGTGAGGGGTT
  TGGGGGCCTGGGGCTTTTCTGTCGGCTTATTAAAATAAACTCGTATGTAATCCTTCTATTTTGATTCGTA
  TATTAAAACCTACCCCCCAAGTAAAGGGAGTGGTGCCTATGAAACCTAATAAAGCCAGTTTCTGAAAATG
  TGCCTGTGTCTGACGTGGTCCTCTTTTCCTCTACCACACACTGACCATTCATTGTACACACCTTTGCTGC
  CTCCTGACAC
• OTI Disclaimer: All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
• Product Components:
  • 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
  • 1 x 100 pmol VP1.5 primer
  • 1 x 100 pmol XL39 primer
• Also Contains: Both mature and minor (star) miRNA can be over expressed from the construct.

Reference Data

• RefSeq: MI0000954
• Synonyms: rno-mir-214
• Locus ID: 100314055