MDA5 (IFIH1) Human shRNA Lentiviral Particle (Locus ID 64135)

CAT#: TL303992V

IFIH1 - Human shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL303992A virus into HEK293 cells. TL303992A virus was prepared using lenti-shRNA TL303992A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL303992A virus into HEK293 cells. TL303992A virus was prepared using lenti-shRNA TL303992A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL303992B virus into HEK293 cells. TL303992B virus was prepared using lenti-shRNA TL303992B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL303992B virus into HEK293 cells. TL303992B virus was prepared using lenti-shRNA TL303992B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL303992C] virus into HEK293 cells. [TL303992C] virus was prepared using lenti-shRNA [TL303992C] and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL303992C] virus into HEK293 cells. [TL303992C] virus was prepared using lenti-shRNA [TL303992C] and TR30037 packaging kit.

• GFP signal was observed under microscope at 48 hours after transduction of [TL303992D] virus into HEK293 cells. [TL303992D] virus was prepared using lenti-shRNA [TL303992D] and TR30037 packaging kit.

• GFP signal was observed under microscope at 48 hours after transduction of [TL303992D] virus into HEK293 cells. [TL303992D] virus was prepared using lenti-shRNA [TL303992D] and TR30037 packaging kit.

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Specifications

Product Data

• Locus ID: 64135
• Synonyms: AGS7; Hlcd; IDDM19; MDA-5; MDA5; RLR-2; SGMRT1
• Vector: pGFP-C-shLenti
• Format: Lentiviral particles
• RefSeq: NM_022168, NM_022168.1, NM_022168.2, NM_022168.3, BC007966, BC046208, BC078180, BC111750, NM_022168.4
• UniProt ID: Q9BYX4
• Summary: IFIH1 encodes MDA5 which is an intracellular sensor of viral RNA that triggers the innate immune response. Sensing RNA length and secondary structure, MDA5 binds dsRNA oligonucleotides with a modified DExD/H-box helicase core and a C-terminal domain, thus leading to a proinflammatory response that includes interferons. It has been shown that Coronaviruses (CoVs) as well as various other virus families, are capable of evading the MDA5-dependent interferon response, thus impeding the activation of the innate immune response to infection. MDA5 has also been shown to play an important role in enhancing natural killer cell function in malaria infection. In addition to its protective role in antiviral responses, MDA5 has been implicated in autoimmune and autoinflammatory diseases such as type 1 diabetes, systemic lupus erythematosus, and Aicardi-Goutieres syndrome[provided by RefSeq, Jul 2020]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).