PEG3 Human shRNA Lentiviral Particle (Locus ID 5178)

CAT#: TL310507V

PEG3 - Human shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL310507A virus into HEK293 cells. TL310507A virus was prepared using lenti-shRNA TL310507A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL310507B virus into HEK293 cells. TL310507B virus was prepared using lenti-shRNA TL310507B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL310507C virus into HEK293 cells. TL310507C virus was prepared using lenti-shRNA TL310507C and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL310507D virus into HEK293 cells. TL310507D virus was prepared using lenti-shRNA TL310507D and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 5178
• Synonyms: PW1; ZKSCAN22; ZNF904; ZSCAN24
• Vector: pGFP-C-shLenti
• Format: Lentiviral particles
• RefSeq: NM_001146184, NM_001146185, NM_001146186, NM_001146187, NM_006210, BC150272, BC037330, BC052616, BC136268, NM_001369722, NM_001369718, NM_001369720, NM_001369723, NM_001369725, NM_001369728, NM_001369729, NM_001369731, NM_001369732, NM_001369733, NM_001369734, NM_001369738, NR_161475, NR_161476, NM_001369717, NM_001369719, NM_001369721, NM_001369724, NM_001369726, NM_001369727, NM_001369730, NM_001369735, NM_001369736, NM_001369737, NM_001369739
• Summary: 'In human, ZIM2 and PEG3 are treated as two distinct genes though they share multiple 5' exons and a common promoter and both genes are paternally expressed (PMID:15203203). Alternative splicing events connect their shared 5' exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. In contrast, in other mammals ZIM2 does not undergo imprinting and, in mouse, cow, and likely other mammals as well, the ZIM2 and PEG3 genes do not share exons. Human PEG3 protein belongs to the Kruppel C2H2-type zinc finger protein family. PEG3 may play a role in cell proliferation and p53-mediated apoptosis. PEG3 has also shown tumor suppressor activity and tumorigenesis in glioma and ovarian cells. Alternative splicing of this PEG3 gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2009]'
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).