Mfsd2a Mouse shRNA Lentiviral Particle (Locus ID 76574)

CAT#: TL514169V

Mfsd2 - Mouse shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL514169A virus into HEK293 cells. TL514169A virus was prepared using lenti-shRNA TL514169A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL514169B virus into HEK293 cells. TL514169B virus was prepared using lenti-shRNA TL514169B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL514169C] virus into HEK293 cells. [TL514169C] virus was prepared using lenti-shRNA [TL514169C] and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL514169D] virus into HEK293 cells. [TL514169D] virus was prepared using lenti-shRNA [TL514169D] and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 76574
• Synonyms: 1700018O18Rik; Mfsd2; NLS1
• Vector: pGFP-C-shLenti
• Format: Lentiviral particles
• RefSeq: BC046793, BC060526, NM_029662, NM_029662.1, NM_029662.2
• UniProt ID: Q9DA75
• Summary: Sodium-dependent lysophosphatidylcholine (LPC) symporter, which plays an essential role for blood-brain barrier formation and function (PubMed:24828044, PubMed:24828040). Specifically expressed in endothelium of the blood-brain barrier of micro-vessels and transports LPC into the brain. Transport of LPC is essential because it constitutes the major mechanism by which docosahexaenoic acid (DHA), an omega-3 fatty acid that is essential for normal brain growth and cognitive function, enters the brain. Transports LPC carrying long-chain fatty acids such LPC oleate and LPC palmitate with a minimum acyl chain length of 14 carbons. Does not transport docosahexaenoic acid in unesterified fatty acid (PubMed:24828044). Specifically required for blood-brain barrier formation and function, probably by mediating lipid transport. Not required for central nervous system vascular morphogenesis (PubMed:24828040). Acts as a transporter for tunicamycin, an inhibitor of asparagine-linked glycosylation.[UniProtKB/Swiss-Prot Function]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).