FBXW7 Human shRNA Plasmid Kit (Locus ID 55294)

CAT#: TL304541

FBXW7 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL304541A virus into HEK293 cells. TL304541A virus was prepared using lenti-shRNA TL304541A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL304541B virus into HEK293 cells. TL304541B virus was prepared using lenti-shRNA TL304541B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL304541C] virus into HEK293 cells. [TL304541C] virus was prepared using lenti-shRNA [TL304541C] and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL304541D] virus into HEK293 cells. [TL304541D] virus was prepared using lenti-shRNA [TL304541D] and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 55294
• Synonyms: AGO; CDC4; FBW6; FBW7; FBX30; FBXO30; FBXW6; hAgo; hCdc4; SEL-10; SEL10
• Vector: pGFP-C-shLenti
• E. coli Selection: Chloramphenicol
• Mammalian Cell Selection: Puromycin
• Format: Lentiviral plasmids
• Kit Components: FBXW7 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 55294). 5µg purified plasmid DNA per construct
  Non-effective 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
• RefSeq: NM_001013415, NM_001257069, NM_018315, NM_033632, NM_001349798, NM_033632.1, NM_033632.2, NM_033632.3, NM_001013415.1, NM_018315.1, NM_018315.2, NM_018315.3, NM_018315.4, NM_001257069.1, BC014503, BC037320, BC059373, BC117244, BC117246, BC143944, NM_001013415.2, NM_018315.5
• UniProt ID: Q969H0
• Summary: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).