AMPK alpha 1 (PRKAA1) Human shRNA Plasmid Kit (Locus ID 5562)

CAT#: TL320461

PRKAA1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL320461B virus into HEK293 cells. TL320461B virus was prepared using lenti-shRNA TL320461B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL320461C] virus into HEK293 cells. [TL320461C] virus was prepared using lenti-shRNA [TL320461C] and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 5562
• Synonyms: AMPK; AMPKa1; AMPK alpha 1
• Vector: pGFP-C-shLenti
• E. coli Selection: Chloramphenicol (34 ug/ml)
• Mammalian Cell Selection: Puromycin
• Format: Lentiviral plasmids
• Kit Components: PRKAA1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 5562). 5µg purified plasmid DNA per construct
  Non-effective 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
• RefSeq: NM_006251, NM_206907, NM_001355028, NM_001355029, NM_001355034, NM_001355035, NM_001355036, NM_001355037, NM_006251.1, NM_006251.3, NM_006251.4, NM_006251.5, NM_206907.1, NM_206907.2, NM_206907.3, BC012622, BC037303, BC048980
• UniProt ID: Q13131
• Summary: The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).