Vitamin D Receptor (VDR) Human shRNA Plasmid Kit (Locus ID 7421)

CAT#: TL320568

VDR - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL320568A virus into HEK293 cells. TL320568A virus was prepared using lenti-shRNA TL320568A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL320568B virus into HEK293 cells. TL320568B virus was prepared using lenti-shRNA TL320568B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL320568C] virus into HEK293 cells. [TL320568C] virus was prepared using lenti-shRNA [TL320568C] and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL320568D] virus into HEK293 cells. [TL320568D] virus was prepared using lenti-shRNA [TL320568D] and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 7421
• Synonyms: NR1I1; PPP1R163
• Vector: pGFP-C-shLenti
• E. coli Selection: Chloramphenicol (34 ug/ml)
• Mammalian Cell Selection: Puromycin
• Format: Lentiviral plasmids
• Kit Components: VDR - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 7421). 5µg purified plasmid DNA per construct
  Non-effective 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
• RefSeq: NM_000376, NM_001017535, NM_001017536, NM_000376.1, NM_000376.2, NM_001017535.1, NM_001017536.1, BC060832, BC060832.1, BC033465, BM685276, BM908433, NM_001364085, NM_001017535.2, NM_001017536.2, NM_000376.3
• UniProt ID: P11473
• Summary: This gene encodes vitamin D3 receptor, which is a member of the nuclear hormone receptor superfamily of ligand-inducible transcription factors. This receptor also functions as a receptor for the secondary bile acid, lithocholic acid. Downstream targets of vitamin D3 receptor are principally involved in mineral metabolism, though this receptor regulates a variety of other metabolic pathways, such as those involved in immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Jun 2018]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).