Srrm4 Mouse shRNA Plasmid (Locus ID 68955)

CAT#: TL504242

Srrm4 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL504242A virus into HEK293 cells. TL504242A virus was prepared using lenti-shRNA TL504242A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL504242B virus into HEK293 cells. TL504242B virus was prepared using lenti-shRNA TL504242B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL504242C] virus into HEK293 cells. [TL504242C] virus was prepared using lenti-shRNA [TL504242C] and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 68955
• Synonyms: 1500001A10Rik; B230202K19Rik; bv; mKIAA1853; nSR100
• Vector: pGFP-C-shLenti
• E. coli Selection: Chloramphenicol (34 ug/ml)
• Mammalian Cell Selection: Puromycin
• Format: Lentiviral plasmids
• Kit Components: Srrm4 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 68955). 5µg purified plasmid DNA per construct
  Non-effective 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
• RefSeq: BC056392, NM_026886, NM_026886.1, NM_026886.2, NM_026886.3, BC024130
• UniProt ID: Q8BKA3
• Summary: Splicing factor specifically required for neural cell differentiation. Acts in conjunction with nPTB/PTBP2 by binding directly to its regulated target transcripts and promotes neural-specific exon inclusion in many genes that function in neural cell differentiation. Required to promote the inclusion of neural-specific exon 10 in nPTB/PTBP2, leading to increased expression of neural-specific nPTB/PTBP2. Also promotes the inclusion of exon 16 in DAAM1 in neuron extracts (PubMed:19737518). Promotes alternative splicing of REST transcripts to produce REST isoform 2 (REST4) with greatly reduced repressive activity, thereby activating expression of REST targets in neural cells (PubMed:21884984). Plays an important role during embryonic development as well as in the proper functioning of the adult nervous system. Regulates alternative splicing events in genes with important neuronal functions (PubMed:25838543).[UniProtKB/Swiss-Prot Function]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).