pCas-Guide-Puro-CRISPRa gene activation vector

CAT#: GE100081

pCas-Guide-Puro-CRISPRa Vector, All-in-one Vector for CRISPR/Cas9 Gene activation with Puro selection marker, dCas9-VP64 and gRNA cloning site.


Reconstitution Protocol

USD 480.00

2 Weeks*

Size
    • 10 ug

Product Images

Specifications

Product Data
Function All-in-one CRISPRa SAM vector
Features
  • BamH I and BsmB I can be used for genomic target sequence cloning
  • gRNA under U6 promoter
  • gRNA is modified with MS2 RNA aptamer in the loops
  • CMV driven dCas9-VP64, which can activate gene expression
  • For robust gene activation, needs to work with pCRISPRa-Enhancer
  • CF3 primer can be used to sequence target sequence after cloning
  • Contains puromycin resistance gene under SV40 promoter
  • Download vector sequence

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.